Journal: Scientific Reports

Article Title: Inhaled sphingosine has no adverse side effects in isolated ventilated and perfused pig lungs

doi: 10.1038/s41598-021-97708-3

Figure Lengend Snippet: ( a , b ) Fluorescence intensity after staining with Cy3-coupled anti-sphingosine ( a ) and autofluorescence intensity without staining as control ( b ). Histological studies demonstrate an accumulation of sphingosine in bronchial epithelial cells after inhalation. EVLP minipig lungs were inhaled with sphingosine at concentrations of 125 µM sphingosine (SPH), 500 µM sphingosine (SPH) and with 0.125% octylglucopyranoside (OGP) as control. The lungs were subjected to bronchoscopy 30 and 150 min after the inhalation, biopsies were fixed in paraformaldehyde, embedded in paraffin and sectioned. Sections were stained with Cy3-coupled anti-sphingosine and without coupling (control). Shown are representative immune stainings. Given is the mean ± SD from 3 sections with 5 visual fields per animal (blinded tests), * p < 0.05, ** p < 0.01, *** p < 0.001, ANOVA.

Article Snippet: Cy3 donkey anti-mouse IgM F(ab) 2 fragments (#715–166-020; Jackson ImmunoResearch) or Cy5-coupled donkey anti-mouse IgM antibody (#715–176-020; Jackson ImmunoResearch) were used as secondary antibodies for visualization.

Techniques: Fluorescence, Staining, Control

Journal: Acta Neuropathologica

Article Title: A glutaminyl cyclase-catalyzed α-synuclein modification identified in human synucleinopathies

doi: 10.1007/s00401-021-02349-5

Figure Lengend Snippet: Cocktails of primary antibodies used for triple labelling immunohistochemistry

Article Snippet: α-Synuclein (Syn1) , 1:3000 , Mouse , BD transduction , Donkey anti-mouse Cy3.

Techniques: Transduction